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portada Nanoscale Imaging and Characterisation of Amyloid-β (Springer Theses)
Type
Physical Book
Publisher
Author
Year
2016
Language
English
Pages
172
Format
Hardcover
ISBN13
9783319395333
Edition No.
1
Categories

Nanoscale Imaging and Characterisation of Amyloid-β (Springer Theses)

Claire Louisa Tinker-Mill (Author) · Springer · Hardcover

Nanoscale Imaging and Characterisation of Amyloid-β (Springer Theses) - Claire Louisa Tinker-Mill

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Synopsis "Nanoscale Imaging and Characterisation of Amyloid-β (Springer Theses) "

This thesis presents a method for reliably and robustly producing samples of amyloid-ss (Ass) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties. Amyloid-ss (Ass) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Ass with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.

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