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portada High-Throughput Screening and Evaluation of Combinatorial Cell Penetrating Peptoid Libraries to Identify Organelle- and Organ-Specific Drug Delivery Molecules
Type
Physical Book
Publisher
Author
Language
English
Format
Paperback
ISBN13
9783736999961

High-Throughput Screening and Evaluation of Combinatorial Cell Penetrating Peptoid Libraries to Identify Organelle- and Organ-Specific Drug Delivery Molecules

Wehl, Ilona Diana Majella (Author) · Lightning Source Inc · Paperback

High-Throughput Screening and Evaluation of Combinatorial Cell Penetrating Peptoid Libraries to Identify Organelle- and Organ-Specific Drug Delivery Molecules - Wehl, Ilona Diana Majella

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Synopsis "High-Throughput Screening and Evaluation of Combinatorial Cell Penetrating Peptoid Libraries to Identify Organelle- and Organ-Specific Drug Delivery Molecules "

Drug delivery systems can increase the pharmacokinetic and pharmacodynamic properties of various therapeutic agents. These systems can ensure a controlled release, improve the specificity of drugs to the target tissue, and therefore decrease side effects to the healthy surrounding tissue. In this work cell penetrating peptoids, which mimic the structure of peptides but exhibit increased stability, were investigated as organelle- and organ-specific carrier molecules. Several fluorescently labelled peptoid libraries, differing for example in length and side-chain composition, were synthesized and investigated with high-throughput screenings in different cell lines. The focus was on the elucidation of structure-function relationships for cellular uptake, intracellular localization and cytotoxicity. Furthermore, the organ targeting of the peptoids was analysed in in vivo studies by using zebrafish as model organism. In addition, the influence of the fluorescent dye on the localization and uptake of the peptoids was investigated.

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