Name: gene transfer and expression protocols
SKU: 3431835
Price: 180.94 EUR
Availability: InStock
Author: murray
ISBN: 9780896031784

portada gene transfer and expression protocols

Type

Physical Book

Publisher

Springer

Author

murray

Language

English

Pages

456

ISBN

0896031780

ISBN13

9780896031784

gene transfer and expression protocols

Murray (Author) · Springer · Physical Book

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Synopsis "gene transfer and expression protocols"

murrays new handbook on gene transfer and expression protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in humanas methods in molecular biology series. each chapter is devoted to a thorough exposition of a single technique. an introduction explains the significance of the protocol and provides background information. a materials section lists all the requirements for the technique discussed. a methods section details the procedure in a step-by-step protocol. a notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. each technique is designed to guarantee optimum results. this volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. topics and techniques include: viral vectors ? reporter genes ? analysis of steady-state level of transcription ? assay for newly initiated transcriptional complexes ? immunocytological techniques ? assay of structure and replication state of transfected genes ? overall strategy for defining regulatory sequences providing the latest techniques in a fast-paced area, gene transfer and expression protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.